Institut für Resistenzforschung und Stresstoleranz
Several project goals have been achieved in Phase 1 and the current Phase 2. We could show that priming efficiency depends on the genetic background of the plant; we identified primable and non-primable genotypes and genomic regions associated with priming. We could also show the suitability of a priming approach under field conditions. In the intended third phase of PrimedPlant we aim to strengthen the translational aspect of the project. We will continue though the quest to understand the mechanism of priming in barley. 1. Obtained data will be used to construct a genomic prediction model for priming efficiency. We will test and adjust this model on experimental field sites on eight different locations. 2. A newly established advanced 10’ set of barley lines, with broad scale of primability, will help to understand the regulation of priming effects. This set will be used to gain applicationoriented information on: i) the agronomic applicability of our priming approach in field trails; ii) priming success under natural occurring pathogen mix, and iii) the possibility of a primingbased strategy against Ramularia collo-cygni. 3. The development of markers for stable QTL based on different priming inducers (AHL, Bacillus) as well as different pathogenic fungi will allow identifying favorable accessions, and therefore support future breeding efforts towards a wider scale of priming scenarios. 4. The modified 15K chip and primer sets will allow faster and more precise breeding progress in terms of priming-associated features.
Federal Ministry of Education and Research