Institut für ökologische Chemie, Pflanzenanalytik und Vorratsschutz
In 2016, 180 barley lines of the GENOBAR-collection were grown, harvested and dried at the IPK-Gatersleben. The material was provided by the federl ex-situ gene bank for agricultural and horticultural crops of the IPK in Gatersleben, Germany. The accessions were collected in Europe, Asia, America, and North Africa. Immediately (t0), germination rate was assayed according to ISTA in all samples and aliquots wer frozen at -80°C. Other aliquots were stored in a a barn and buried in soil. Additionally, eight genotypes were stored under contemporary and suboptimal gene bank standards. Germination rate was determied annually in the barn stored material to estaimate when 50% germination was reached. In early 2023, the germination rate was found to be 35% in a representative mixed sample. Now (spring 2023), germination rate should be assayed in the seeds stored in the two environments (IPK) and aliquots should be frozen at -80°C. All material (t0, barn, soil) should be analyzed using high resolution mass spectrometry and different methods (JKI) to study the process of ageing in the cereal seed. Preliminary studies have been conducted (BLE 04/13-14NZL, FoPro JKI-ÖPV-08-1403, Riewe et a., 2017, Wiebach et al. 2020). Metabolites should be identified that accumulate during ageing. Critical phsiological processes during ageing should be unravelled. Protective metabolites should be investigated. For all traits, regulatory loci/genes/polymorphisms should be found in this completely genotyped collection. The results of this study should extend our knowledge on seed ageing at the genetic and molecular level and help to keep germinability of seeds up wherever relevant (agriculture/conservation/utilization), either by breeding or optimal storage.References:Pasam, R. K., R. Sharma, A. Walther, H. Özkan, A. Graner and B. Kilian (2014). "Genetic Diversity and Population Structure in a Legacy Collection of Spring Barley Landraces Adapted to a Wide Range of Climates." PLOS ONE 9(12): e116164.Riewe, D., J. Wiebach and T. Altmann (2017). "Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS." Plant Physiol 175(2): 600-618.Wiebach, J., M. Nagel, A. Börner, T. Altmann and D. Riewe (2020). "Age-dependent loss of seed viability is associated with increased lipid oxidation and hydrolysis." Plant, Cell & Environment 43(2): 303-314.
Federal Ministry of Food and Agriculture
