The pre-competitive research and prototype demonstration in TERTIUS utilizes the wheat genome
sequence as well as knowledge gained from fundamental research on cellular mechanisms of stress
response to systematically screen the gene pool of wheat for valuable gene variants and to improve the
drought tolerance of elite breeding material through precision breeding approaches. The overall objective of
TERTIUS is to develop wheat prototypes with optimized root performance, improved water-use efficiency
and good baking quality, which are characterized by stable and high grain yield under drought stress. In
order to achieve breeding progress in these traits, a sufficiently high genetic variation in the breeding
material is a basic requirement. TERTIUS aims to increase the genetic variation of the root system in winter
wheat by using wheat-rye translocations. In the genetic background of a high-performance German winter
wheat translocation line, we will develop genotypes with improved nutrient and water absorption and high
yield stability under drought stress. We characterize the physiology of the stress response of such
genotypes by implementing innovative methods of phenotyping root architecture, shoot growth and
architecture, and the performance of the photosynthetic apparatus. We assess the drought stress tolerance
of wheat genotypes with optimized root systems in representative target environments under farm
conditions and analyze the genetic architecture of winter wheat water use efficiency. An association study
will close a current knowledge gap and provide basic insights into the genetic architecture of water use
efficiency of wheat with translocation segments under the influence of drought stress.