Overall objective of the project:
The project aims at the characterization of the resistance of released cultivars and of new breeding strains accepted for official testing. The tests of resistance are carried out (a) in field trials with four replications after artificial inoculation with a mixture of virulent rust-isolates. The disease severity is evaluated at least three times at intervals of one week by recording the percentage of the leaf area covered with pustules within one plot. Multiple readings allow the calculation of a disease progress curve for each genotype tested. In addition, the genotypes are tested (b) at the seedling stage with two replications. For this purpose three to six single spore isolates are used with defined and different virulence patterns. These tests provide information about seedling resistance as well as about infection types. In the case of isolate x genotype interactions resistance genes can be identified. The results contribute to the decision of variety release of the Federal Seed Board.
Overall objective of the project:
Characterization of cultivars and candidates of winter and spring barley for resistance to Puccinia hordei; identification of qualitative resistance in seedling tests with defined isolates and of quantitative resistance by determination of the area under.
Overall objective of the project:
The project aims at the detection of new species potentially endangering crop production and the establishment of a data base to get information on the influence of global climatic and environmental changes on the epidemiology of aphids. Thereby risk assessment for aphid pests and aphid transmitted plant viruses are facilitated providing the basis for the reduction of insecticide sprayings. Flight activity of aphids is monitored in a suction trap and a yellow water trap. Using stadardized traps enable the establishment of a EU-wide database. By determination of the trapped aphid species their flight activity is characterised and new species detected. Comparison of the flight activity and the natural occurrence of aphids in field plots allows a more accurate estimation of the resistance degree of wheat and barley genotypes.
Overall objective of the project:
For the evaluation of genetic resources with respect to resistance the availability of defined pathogenic isolates is a prerequisite. The collection of pathogens has to be updated permanently. New strains must be isolated and classified according to their virulence and race specifity. Special methods for long time storage have to be applied to maintain the characteristics and to have pathogenic isolates available for epidemiological and resistance studies within the BAZ as well as for scientific and commercial institutions.
Cooperation partner: ZADI-Zentralstelle für Agrardokumentation Gemeinschaft zur Förderung von Pflanzeninnovation e. V.
Overall objective of the project:
The project has been conducted since 2001 by BAZ, ZADI, GFP and numerous breeding companies. It contributes to establishment of durable structures for the evaluation of plant genetic resources in breeding and research. By that means the efficiency of German evaluation activities will be increased and the durable exploitation of plant genetic ressources in breeding and research will be guaranteed. Based on the establishment of a cooperative network for the evaluation of plant genetic resources of main cereal crops, i.e., wheat and barley, a multilocal evaluation systeme will be established. To some extent molecular markers will be integrated in the evaluation of genetic resources. The generated evaluation data are transferred to the national system of documentation for plant genetic resources and are accessible for all participants and later on to the public.
Overall objective of the project:
The aim of the project is to identify resistance against the two aphid species Myzus persicae and Aphid fabae and thus to create the basis for a later genetic mapping of the resistance. This will be achieved with the help of a broad methodological approach, with entomological methods clearly in the foreground. In addition, a comparison of the results of the aphid species investigated should show whether the resistances observed here is general or whether there is a specific resistance based on an interaction of aphid species-specific effectors (Rodriguez et al., 2017) and plant R proteins. Beside this, the study aims to assess different entomological methods in terms of informative value, comparability and cost-effectiveness with regard to an economically-based screening. It should also be considered, which influence environmental factors and cultivation conditions (greenhouse vs. field) have on some of the methods as well as on the tested resistance.
Cooperation partner: Justus-Liebig-Universität Gießen Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung Limagrain GmbH ABiTEP GmbH
Overall objective of the project:
The combination of priming-responsive genomes with resistance- or priming-inducing strains, consortia or soil microbiome has great potential. It is the key innovation of our approach, and we are confident that it will lead to novel approaches. In the second phase (PrimedPlant2), we intent to implement the finding from the first phase and transfer them into field conditions. The aim is to prove their applicability for growers on the one hand (direct use of the biologicals) and for the breeding companies, on the other (identified markers and QTLs along with the barley lines).
Our principal effort will be a field experiment conducted on two different sites in Germany, in which the chosen set of seven barley accession (7’set) will be tested for their priming effects. This experimental setup requires the expertise of all consortium members: breeding, microbiology, plant physiology, genetics and phytopathology.
The information on QTLs identified upon priming for enhanced resistance against P. hordei will be compared to QTL associated with priming for P. teres-resistance, i.e. to assess the information whether the respective QTLs are pathogen specific or universally involved in the priming phenomenon. This information will be extended with transcriptome and metabolome analyzes. An additional effect of the identified microbiota on root growth may have positive effects on the nutrient uptake, also under unfavorable conditions, such as drought stress.
Cooperation partner: Institut für Pflanzenbau und Bodenkunde (JKI)
Overall objective of the project:
In this project, the expertise of the three institutes SF (process-based plant growth models), BI (biological plant protection) and RS (drought stress tolerance, TST) will be combined in order to (i) identify parameters which, when combined, enable the highest possible TST in barley and which are worth selecting in future breeding programmes, (ii) identify biological preparations that support tolerance properties, (iii) investigate possible effects of TST of an acession on their susceptibility to powdery mildew, and (iv) evaluate at which developmental stages TST properties in barley contribute significantly to yield stabilisation. The synergy effects and knowledge about possible negative correlations between TST and susceptibility to pathogens will thus be used to develop important breeding parameters for the development of resistant and efficient varieties and to adapt them to climate changes.